Binding of F(ab′)2 of Normal Human IgG to Human Lymphocytes

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Abstract
The presence of immunoglobulins (Ig) on the surface of B lymphocytes in humans is well established. However, there has been considerable controversy concerning the proportion of lymphocytes in the peripheral blood bearing on their surface Ig of the IgG class. These different results may be due to variations in techniques, potency, and specificity of the reagents or to the presence of various populations of cells displaying surface IgG under particular conditions. The IgG-bearing cell population probably consists of cells possessing endogenously synthesized IgG present on the membrane and cells having Ig adsorbed onto their surface. Most of the IgG binding has been ascribed to the presence of Fc receptors on the cell surface (1). We have found, however, that part of the IgG bound to cells may be attributed to molecules bound through their Fab part. Materials and Methods. Antigens.