Separation of Lymphocytes from Disaggregated Mouse Malignant Neoplasms by Sedimentation in Gradients of Ficoll in Tissue Culture Medium

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Abstract
A method is reported for separating lymphocytes from solid tumors. After the sequential use of columns of glass beads, velocity (isokinetic) sedimentation, and isopycnic sedimentation, lymphocytes were obtained in 76.3 to 96.8% purity from disaggregated tumors. When isopycnic sedimentation was omitted, lymphocytes were obtained in 58.4 to 95.1% purity.