Cytokinetic properties of asynchronous and cytosine arabinoside perturbed murine tumors measured by simultaneous bromodeoxyuridine/DNA analyses

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Abstract
This paper describes the determination of cytokinetic properties of asynchronous and cytosine arabinoside‐(Ara‐C) treated KHT tumors growing in vivo using the bromodeoxyuridine (BrdUrd)/DNA analysis technique. The cytokinetic properties of asynchronously growing tumors were estimated by computer analysis of sequential BrdUrd/DNA distributions measured at 2‐ to 3‐h intervals after administration of a single i.p. injection of BrdUrd. The cytokinetic properties of the AraC‐treated tumors were estimated by computer analyysis of BrdUrd/DNA distribution sured at 2‐ to 3‐h intervals after Ara‐C treatment. BrdUrd was injected 30 min prior to tumor harvest. The cytokinetic properties of the cells rendered nonclonogenic by Ara‐C were followed in BrdUrd/DNA distributions measured at 2‐ to 3‐h intervals after Ara‐C treatment of tumors that were labeled with BrdUrd 30 min prior to Ara C injection. The Gl‐, S‐, and G2M‐phase durations were estimated to be 7.6, 10.9, and 2.0 h prior to Ara‐C; decreasing to 1.2, 4.1, and 1.4 after Ara‐C. The growth fraction was estimated to be 0.8 prior to Ara‐C. Complete recruitment of the normally noncycling subpopulation was observed after Ara‐C treatment. Ara‐C‐killed cells were removed from the tumor within 24 h following Ara‐C injection. These cytokinetic properties were similar to those reported in other studies.