Construction of φ80 dhisCarryingSalmonella typhimuriumHistidine Operon Mutations

Abstract
Starting with a previously isolated F′hisepisome and φ80 dhis immλcI857transducing phage, we have constructed recombinant elements bearing previously isolatedhismutations fromSalmonella typhimurium. These phages were constructed as sources of deoxyribonucleic acid for in vitro biochemical experiments on gene regulation. The manipulation of genes betweenS. typhimuriumandEscherichia colidescribed here may be useful in studying otherS. typhimuriumoperons.