NADPH-cytochrome P-450 oxidoreductase gene organization correlates with structural domains of the protein
- 1 October 1990
- journal article
- research article
- Published by American Chemical Society (ACS) in Biochemistry
- Vol. 29 (42) , 9814-9818
- https://doi.org/10.1021/bi00494a009
Abstract
CDNA clones to rat liver NADPH-cytochrome P-450 oxidoreductase were used to isolate genomic clones from a Wistar-Furth inbred rat genomic DNA library. Fifteen exons containing the coding region and 3''-nontranslated segment of the P-450 reductase gene were identified, spanning 20 kilobases of DNA contained in 3 .lambda.-Charon 35 clones. The organization of this single copy gene reveals a general correspondence between exons and structural domains of the protein, with the segment responsible for anchoring the reductase to the microsomal membrane and several segments involved in FMN, FAD, and NADPH binding encoded by discrete exons.This publication has 28 references indexed in Scilit:
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