A RAPID METHOD FOR THE ESTIMATION OF PROSTAGLANDIN 15‐HYDROXYDEHYDROGENASE ACTIVITY AND ITS APPLICATION TO PHARMACOLOGY

1 August 1976

journal article
research article
Published by Wiley in British Journal of Pharmacology

Vol. 57 (4) , 589-597
https://doi.org/10.1111/j.1476-5381.1976.tb10389.x

Abstract

A novel double-isotope assay for prostaglandin 15-hydroxydehydrogenase (PGDH) is described. 2 The assay is simple, rapid, precise, completely specific for PGDH, and free from artefacts. The results obtained correlate well with a conventional assay. 3 The method is versatile, being applicable to estimations of enzyme activity in cell-free systems as well as perfused organs, and is well suited to the assay of PGDH inhibitors.

Keywords

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