THE VITAMIN D ASSAY OF THE "REFERENCE COD-LIVER OIL"

12 October 1934

journal article
research article
Published by American Association for the Advancement of Science (AAAS) in Science

Vol. 80 (2076) , 342
https://doi.org/10.1126/science.80.2076.342

Abstract

Preliminary feeding trials showed that each chick required between 80 and 135 I. U. based on the total length (4 weeks) of the A.O.A.C. method of feeding test to produce satisfactory calcification as observed by the criteria of normal growth and an average bone ash content in excess of 45%. Further support is presented for the theory that the vitamin D requirement of the chick for the antirachitic substance of cod-liver oil is enormous when compared with that of the rat. The reference cod-liver oil, containing 95 I. U. per gm., was found to be considerably poorer as a source of antirachitic activity than a number of commercial poultry cod-liver oils which had been assayed.

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