Human Cytomegalovirus. Observations of Intracellular Lesion Development as Revealed by Phase Contrast, Time-Lapse Cinematography

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Abstract
Human fetal fibroblasts (WI-38) growing in Rose chambers were infected with the Rib strain of human cytomegalovirus. Time-lapse cinematography was employed for the first time to observe cytopathology in living, infected cells. In the sequence of events, the infected cell first contracted and then developed a cytoplasmlc lesion composed of aggregated granules and vacuoles, which eventually occupied a paranuclear position. Inclusion bodies subsequently formed in the nucleus. As the intranuclear Inclusions matured, the nucleus rotated about the cell and the cytoplasmlc inclusion became more prominent before the cell exploded and distinctive cellular morphology disappeared.