Abscission of Citrus Leaf Explants

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Abstract
The role of .alpha.-naphthaleneacetic acid (NAA) in the control of abscission in Citrus (C. sinensis L. Osbeck) leaf explants and its conjugation were studied in non-aged and 24 h aged explants. Dipping non-aged explants in 1.5 .mu.M NAA for 15 min immediately after excision did not delay abscission whereas 150 .mu.M NAA effectively delayed it. As incubation time was prolonged up to 24 h after excision, the delaying effect of both concentrations gradually increased. In general, both concentrations did not delay abscission when applied to 24 h aged explants held for an additional period of up to 24 h. The uptake and conjugation of 14C-NAA to glucose and aspartic acid were similar in petiole, abscission zone and leaf blade of non-aged and aged tissues, for all NAA concentrations. No correlation was established between the kinetics of abscission and the rate of conjugation in the abscission zone.