Deoxyribonucleic Acid Relationships Among Hydrogen-Oxidizing Strains of the Genera Pseudomonas, Alcaligenes, and Paracoccus

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Abstract
Optical determination of DNA-DNA reassociation kinetics was applied to the classification of 32 selected strains of H2-oxidizing bacteria belonging to the genera Pseudomonas, Alcaligenes and Paracoccus. The renaturation studies revealed a high intraspecies DNA homology for some strains of the species P. palleronii, P. pseudoflava and A. paradoxus, supporting former taxonomic concepts of different authors. The 12 denitrifying strains belonging to the genus Paracoccus were interrelated at various levels of percent degree of binding and at least 2 clusters of very high genome-DNA relatedness were found. DNA-DNA reassociation kinetics were also used for the calculation of the average molecular weight of genome DNA. The genome MW of the H2-oxidizing bacteria investigated in this study ranged from 3-5 .times. 109.