The Biosynthesis of Some Isothiocyanates and Oxazolidinethiones in Rape (Brassica campestris L.)

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Abstract
The incorporation of the radioactivity from acetate-1-14C, acetate-2-14C, DL-methionine-l-14C, DL-methionine-2-14c, DL-methionine-3,4-14C, DL-homomethionine-2-14C, DL-allyl-glycine-2-14c, and DL-2-amino-5-hydroxyvalerate-2-14C into the aglycones of progoitrin, gluconapin, and glucobrassicanapin of maturing rape plants (B. campestris) was investigated. Radioactivity from DL-methionine-2-14c, DL-methionine-3,4-14c, DL-homomethionine-2-14C, and acetate-2-14C were incorporated into the 3 major thiogluco-sides. The other organic compounds were poorly incorporated except for DL-allylglycine-2-14c into glucobrassicanapin. The results obtained suggest that the rape plant can synthesize amino acids bythe condensation of acetate (as acetyl CoA) to [alpha]-keto acids to yield a homologue of the original amino acid. These newly formed amino acids are then employed to synthesize the 3 major thioglucosides.