Post-Irradiation Thymocyte Regeneration After Bone Marrow Transplantation Ii. Physical Characteristics of Thymocyte Progenitor Cells

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Abstract
Bone marrow cells [BMC] were separated according to buoyant density, velocity sedimentation and cell surface charge. Fractionated (C3H .times. AKR)F1 [mouse] BMC were transplanted into lethally-irradiated C3H recipients. In all fractions, the CFU [hemopoietic stem cell] content and the capacity to restore the thymus cell population were determined. For all the physical parameters tested, thymocyte progenitor cells show the same distribution as CFU. The relationship between number of thymocyte progenitor cell and CFU number is dependent on density. Bone marrow progenitors of phytohemagglutinin responsive cells are of low buoyant density and show a distribution which resembles the distribution of Thy 1 positive cell progenitors. After transplantation of large numbers of bone marrow cells into irradiated mice, no significant change in the CFU content of the thymus was observed.