Chloretone as a Trematode Relaxer, and Its Use in Mass-Collecting Techniques

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Abstract
A soln. of 2 g. of Chloretone in 500 ml. of water is used to narcotize both digenetic and monogenetic trematodes. (chloretone has been used successfuly by the author on various Acanthocephala, Cestoda, Copepoda and Nematoda). For use on fresh-water ectoparasites the solvent is fresh-water, for marine ectoparasites and all endoparasites the solvent is filtered sea-water (where sea-water is not available a suitable physiological saline solvent may be used). The use of this soln. in both small-scale and mass-collecting technics is discussed and procedures for both Monogenea and Digenea are presented. This technic has been tested on at least 8 genera of digenetic trematodes and found successful. It apparently works well on all Monogenea. Chloretone-water used as a relaxer results in specimens which are more nearly normal in shape and arrangement of external features and internal organs than those obtained by pressure-fixation technic. The consistent use of a chemical relaxer such as this in a constant soln. should result in more normal and accurate results in morphological studies of all trematodes to which the method can be applied.

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