PRODUCTION OF COLICINS-V-ET-V2 INVITRO AND INVIVO - STUDY OF THEIR INHIBITORY ACTION OF PHAGOCYTOSIS BY PERITONEAL MACROPHAGES

  • 1 January 1977
    • journal article
    • research article
    • Vol. 36  (4) , 307-316
Abstract
In recent years, a possible relationship between pathogenicity and colicinogeny in some Escherichia coli colicin V-producing strains was inferred. A simple in vitro method was elaborated for production of colicin V free of large, non-dialyzable macromolecules and presumably of bacterial endotoxin. This allows study of effects of colicin V in vivo without an undesirable added physiological response of the experimental animal to endotoxin. All Col V+ strains studied displayed a greater ability to survive in the peritoneal cavity of mice than the Col V- strains. Colicin V was detected in peritoneal fluids of mice injected with Col V+ strains. Phagocytosis by peritoneal macrophages seemed to be inhibited in vitro in the presence of colicin V. Colicin V is not toxic in vivo in low concentration after i.p. or i.v. injection, but it may favor the multiplication and invasiveness of the strains that produce it.

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