Determination of Lecithin: Cholesterol Acyltransfer in Human Blood Plasma
- 1 January 1971
- journal article
- research article
- Published by Taylor & Francis in Scandinavian Journal of Clinical and Laboratory Investigation
- Vol. 27 (1) , 21-27
- https://doi.org/10.3109/00365517109080184
Abstract
A method for determining lecithin: cholesterol acyltransferase (LCAT) activity is presented. Trace amounts of labelled cholesterol are added to serum or plasma. During a preincubation time in which the LCAT is inhibited by a disulphide, the tracer equilibrates with endogenous lipoprotein cholesterol. The enzyme is reactivated by excess of thiol. The presented method seems to be a good measure for the in vivo blood plasma cholesterol esterification rate as only autologous, non-heated lipoproteins are used in the assay.Keywords
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