A simplified method of generating transgenic Xenopus
- 15 February 2000
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 28 (4) , 12e-12
- https://doi.org/10.1093/nar/28.4.e12
Abstract
Currently transgenic frog embryos are generated using restriction-enzyme-mediated integration (REMI) on decondensed sperm nuclei followed by nuclear transplantation into unfertilized eggs. We have developed a simplified version of this protocol that has the potential to increase the numbers of normally developing transgenic embryos.Keywords
This publication has 5 references indexed in Scilit:
- Mammalian Transgenesis by Intracytoplasmic Sperm InjectionScience, 1999
- Metamorphosis is inhibited in transgenic Xenopus laevis tadpoles that overexpress type III deiodinaseProceedings of the National Academy of Sciences, 1999
- Transgenic Xenopus embryos from sperm nuclear transplantations reveal FGF signaling requirements during gastrulationDevelopment, 1996
- Interactions between Xwnt-8 and Spemann organizer signaling pathways generate dorsoventral pattern in the embryonic mesoderm of Xenopus.Genes & Development, 1993
- Persistence and replication of plasmid DNA microinjected into early embryos of Xenopus laevisDevelopmental Biology, 1988