Regulation of divalent metal transporter expression in human intestinal epithelial cells following exposure to non‐haem iron
- 24 February 2005
- journal article
- Published by Wiley in FEBS Letters
- Vol. 579 (9) , 1923-1929
- https://doi.org/10.1016/j.febslet.2005.02.035
Abstract
A number of regulatory factors including dietary iron levels can dramatically alter the expression of the intestinal iron transporter DMT1. Here we show that Caco‐2 cells exposed to iron for 4 h exhibited a significant decrease in plasma membrane DMT1 protein, though total cellular DMT1 levels were unaltered. Following biotinylation of cell surface proteins, there was a significant increase in intracellular biotin‐labelled DMT1 in iron‐exposed cells. Furthermore, iron‐treatment increased levels of DMT1 co‐localised with LAMP1, suggesting that the initial response of intestinal epithelial cells to iron involves internalisation and targeting of DMT1 transporter protein towards a late endosomal/lysosomal compartment.Keywords
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