Cellular localization of substance P‐ and neurokinin A‐encoding preprotachykinin mRna in the female rat brain

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Abstract
To determine the locations of neurons in the rat brain expressing substance P and neurokinin A mRNA, we performed in situ hybridization with a radiolabeled cRNA probe that was complementary to α-, β-, and γ-preprotachykinin mRNA. Several types of controls indicated specificity of the labeling. Brain regions containing many labeled neurons include the anterior olfactory nucleus, layer II of the olfactory tubercle, the islands of Calleja, the nucleus accumbens, the caudate-putamen, portions of the amygdala and hypothalamus, the medial habenular nucleus, nuclei of the pontine tegmentum, several raphe nuclei, several portions of the reticular formation, and the nucleus of the solitary tract. Less frequent labeled neurons were also found in many other regions of the brain. These results extend many previous immunocytochemical studies of the locations of neurons containing immunoreactive substance P, neurokinin A, and neuropeptide K.