Attempts to Utilize Mosquito Larvae in a Bioassay1 Method for Insecticide Residues in Animal Products

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Abstract
A method of detecting insecticide contamination of milk and meat by bioassay with mosquito larvae is descr. Tests were made with butterfat containing one of the chlorinated hydro-carbon insecticides[long dash]DDT, TDE, methoxychlor, lindane, chlordane, and toxaphene[long dash]against fourth-instar larvae of Aedes aegypti. Some constituent of emulsified butterfat rendered all these insecticides nontoxic to the larvae. Treating butterfat with sulfuric acid did not remove all the substances that interfered with the toxicity of DDT and TDE, but did eliminate the ingredient that reduced the toxicity of lindane. Toxaphene and chlordane were not sufficiently toxic to Aedes aegypti larvae to be detected by the method that was effective for lindane. In the tests with beef fat, it appeared that acid treatment of larger quantities of chlordane-contaminated beef fat might be utilized in a bioassay with Anopheles quadrimaculatus larvae.

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