Inhibition of bacterial growth by pure ozone in the presence of nutrients

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Abstract
Achromo-bacter and Pseudomonas were more resistant to ozone than Bacillus subtilis. staphylococci, and other bacteria studied. Yeasts and molds were about as susceptible as bacteria. Higher concns. were required to arrest established growth, than to inhibit growth when ozone was admitted and inoculation performed at the same time. More ozone was necessary to inhibit growth at 20[degree] C than at 0[degree]C. A low concn. of ozone would kill bacteria in water (10 ppm.), somewhat more was required to inhibit cul- tures in synthetic media, considerably more to inhibit cultures in nutrient broth, and still higher concns. to inhibit cultures on nutrient agar (several thousand ppm.). Media treated with ozone were bleached, had a low pH, and would support slow and delayed growth if the pH was readjusted before inoculation. Inhibitory concns. were higher than people can tolerate, and spoil food to , which they are applied. Evidence is presented that ozone destroys or interferes with dehydrogenating enzymes of bacteria.

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