Determination of the calcium entry blocker verapamil in plasma by capillary gas chromatography with on‐column injection

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Abstract
A specific, sensitive, and accurate assay for quantitation of verapamil has been developed. The method involves a single extraction step with n‐heptane, followed by evaporation at room temperature under nitrogen. 0.1 μl of the extract was injected into a capillary column coated with crosslinked 5% phenylmethylsilicone. The column separated verapamil, norverapamil, and its internal standard within 15 minutes using temperature programming from 90 to 290°C. The lower limit of detection was 1 ng/ml for verapamil. The calibration curve was linear in the concentration range (5–300 ng/ml). Plasma concentration data from dogs receiving intravenous verapamil infusion are presented.

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