A Lotus japonicus Nodulation System Based on Heterologous Expression of the Fucosyl Transferase NodZ and the Acetyl Transferase NolL in Rhizobium leguminosarum
Open Access
- 1 April 2000
- journal article
- Published by Scientific Societies in Molecular Plant-Microbe Interactions®
- Vol. 13 (4) , 475-479
- https://doi.org/10.1094/mpmi.2000.13.4.475
Abstract
Heterologous expression of NodZ and NolL proteins in Rhizobium leguminosarum bv. viciae led to the production of acetyl fucosylated lipo-chitin oligosaccharides (LCOs), indicating that the NolL protein obtained from Mesorhizo-bium loti functions as an acetyl transferase. We show that the NolL-dependent acetylation is specific for the fucosyl penta-N-acetylglucosamine species. In addition, the NolL protein caused elevated production of LCOs. Efficient nodulation of Lotus japonicus by the NodZ/NolL-producing strain was demonstrated. Nodulation efficiency was further improved by the addition of the ethylene inhibitor L-α-(2-aminoethoxyvinyl) glycine (AVG).Keywords
This publication has 25 references indexed in Scilit:
- The nolL Gene from Rhizobium etli Determines Nodulation Efficiency by Mediating the Acetylation of the Fucosyl Residue in the Nodulation FactorMolecular Plant-Microbe Interactions®, 1999
- Mass spectrometric analysis of lipo-chitin oligosaccharides-Signal molecules mediating the host-specific legume-rhizobium symbiosisMass Spectrometry Reviews, 1998
- A Rhizobium leguminosarum Biovar trifolii Locus Not Localized on the Sym Plasmid Hinders Effective Nodulation on Plants of the Pea Cross-Inoculation GroupMolecular Plant-Microbe Interactions®, 1997
- NodZ of Bradyrhizobium extends the nodulation host range of Rhizobium by adding a fucosyl residue to nodulation signalsMolecular Microbiology, 1996
- Novel and Complex Chromosomal Arrangement ofRhizobium/otiNodulation GenesMolecular Plant-Microbe Interactions®, 1996
- Structural identification of the iipo‐chitin oligosaccharide nodulation signals of Rhizobium lotiMolecular Microbiology, 1995
- Host Specificity ofRhizobium leguminosarumis Determined by the Hydrophobicity of Highly Unsaturated Fatty Acyl Moieties of the Nodulation FactorsMolecular Plant-Microbe Interactions®, 1995
- Structural identification of metabolites produced by the NodB and NodC proteins of Rhizobium leguminosarumMolecular Microbiology, 1994
- Resistance to nodulation of cv. Afghanistan peas is overcome by nodX, which mediates an O‐acetylation of the Rhizobium leguminosarum lipo‐oligosaccharide nodulation factorMolecular Microbiology, 1993
- Detection and Separation ofRhizobiumandBradyrhizobiumNod Metabolites Using Thin-Layer ChromatographyMolecular Plant-Microbe Interactions®, 1992