Solubilisierung von "High Potential" Cytochrom b-559 aus Spinat-Chloroplasten
Open Access
- 1 October 1975
- journal article
- Published by Walter de Gruyter GmbH in Zeitschrift für Naturforschung C
- Vol. 30 (9-10) , 628-633
- https://doi.org/10.1515/znc-1975-9-1012
Abstract
1. Ethanol-extracted spinach chloroplasts were carefully sonified in a mixture of buffered urea, TRITON X-100 and dithiothreitol. This procedure solubilized the membrane-bound b-type cytochromes 559 and 563 to approx 90%. The crude extract contained both the high-potential (HP) and the low-potential form (LP) of cytochrome b-559 in a molar ratio of 1:1. 2. Strong sonification doubled the amount of low potential cytochrome b-559 in the extract. Therefore, it is assumed that Cyt b-559 HP was completely converted into the low-potential form. 3. Cyt b-563 was solubilized in the same concentration by both methods. 4. In the same medium (see No. 1), cytochrome f (= Cyt c-554) was dissolved by strong sonification only and is then essentially present in the extract in a dithionite-reducible form.Keywords
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