MODIFICATION OF BOVINE PANCREATIC RIBONUCLEASE A WITH PYRIDOXAL 5‘‐PHOSPHATE. ISOLATION AND IDENTIFICATION OF DERIVATIVES
- 1 September 1975
- journal article
- Published by Wiley in International Journal of Peptide and Protein Research
- Vol. 7 (5) , 379-387
- https://doi.org/10.1111/j.1399-3011.1975.tb02456.x
Abstract
Bovine pancreatic ribonuclease A was allowed to react with pyridoxal 5′-phosphate at pH 8 and 4°. After reduction with sodium borohydride, the principal products formed in the initial stages of modification were separated by successive chromatography on CM-cellulose and SP-Sephadex. The isolated derivatives were identified as Nα-(P-pyridoxyl)-Lys-1-, Ne-(P-pyridoxyl)-Lys-7-, and Ne-(P-pyridoxyl)-Lys-41-ribonuclease A. These results are interpreted in terms of the specificity of pyridoxal-P as a protein reagent.Keywords
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