Use of dimethylformamide–sulphur trioxide complex as a sulphating agent of tyrosine

Abstract
Dimethylformamide–sulphur trioxide (DMF–SO3) complex was found to be more suitable for tyrosine sulphation than pyridine–sulphur trioxide (Pyr–SO3) complex, the most commonly used sulphur trioxide complex for sulphation. The work-up after sulphation using DMF–SO3 was also easier than with Pyr–SO3. The usefulness of DMF–SO3 complex was demonstrated through the synthesis of two tyrosine sulphate [Tyr(SO3H)]-containing peptides, leucine-enkephalin sulphate and leucosulfakinin-II.

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