Two novel brain proteins, CaBP33 and CaBP37, are calcium‐dependent phospholipid‐ and membrane‐binding proteins
- 12 March 1990
- journal article
- Published by Wiley in FEBS Letters
- Vol. 262 (1) , 72-76
- https://doi.org/10.1016/0014-5793(90)80157-e
Abstract
Two acidic Ca2+ ‐binding proteins (CaBP33 and CaBP37) purified from bovine brain have been characterized in terms of immunological properties, heat‐sensitivity, electrophoretic mobility, and Ca2+‐dependent binding to negatively charged phospholipids and to brain membranes. They were induced to bind to membranes by homogenization of brain tissue in the presence of CaCl2. The membrane‐bound CaBP33/CaBP37 mixture resisted extraction with detergents and was solubilized with high concentrations of EGTA/KC1. However, apparent Ca2+‐independent binding of the two proteins to membranes seemed to occur as well. This latter fraction of membrane‐bound CaBP33 and CaBP37 could be solubilized with Triton X‐100, indicating that brain membranes normally contain the two proteins as intrinsic components.Keywords
This publication has 19 references indexed in Scilit:
- The annexin family of calcium-binding proteins: Review articleCell Calcium, 1989
- Calcium-dependent phospholipid- (and membrane-) binding proteinsBiochemistry, 1988
- Isolation and characterization of two novel calcium‐dependent phospholipid‐binding proteins from bovine lungFEBS Letters, 1988
- Characterization of Calelectrin, a Ca2+‐Binding Protein Isolated from the Electric Organ of Torpedo marmorataJournal of Neurochemistry, 1985
- Mechanism of action of S-100 protein(s) on brain microtubule protein assemblyBiochemical and Biophysical Research Communications, 1984
- Isolation of mammalian calelectrins: a new class of ubiquitous calcium(2+)-regulated proteinsBiochemistry, 1984
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970