O‐Linked neutral sugar chains of porcine zona pellucida glycoproteins
- 1 June 1993
- journal article
- Published by Wiley in European Journal of Biochemistry
- Vol. 214 (3) , 763-769
- https://doi.org/10.1111/j.1432-1033.1993.tb17978.x
Abstract
The O-linked sugar chains were liberated from porcine zona pellucida glycoproteins by alkaline-borohydride treatment and the structures of neutral oligosaccharides were determined. The major oligosaccharides had the following structures with type-1 cores, (Gal beta 1-->4GlcNAc beta 1-->3)1 3-Gal bdta 1-->3N-acetylgalactosaminitol (54%) and GlcNAc beta 1-->3(Gal beta 1-->4GlcNAc beta 1-->3)0-1Gal beta 1-->3N- acetylgalactosaminitol (16%). Approximately 6% of the oligosaccharides had the structure with a type-3 core, Gal beta 1-->4GlcNAc beta 1-->3N-acetylgalactosaminitol. Oligosaccharides having alpha-galactose (2%) and beta-GalNAc (4%) at their non-reducing termini were also found as minor components.Keywords
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