Axenic Cultivation of Entamoeba histolytica
- 4 August 1961
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 134 (3475) , 336-337
- https://doi.org/10.1126/science.134.3475.336
Abstract
Entamoeba histolytica (National Institutes of Health strain 200) has been maintained in axenic culture through 50 transfers over a period of 6 months. The medium used is diphasic and contains a cell-free extract of chick embryo which is essential for growth. Inocula of 50,000 amoebas yield 150,000 to 200,000 organisms in 72 hours of incubation at 35°C. An extract prepared from boiled embryos supports growth equally well.Keywords
This publication has 3 references indexed in Scilit:
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- The Rate of Reproduction of Entamoeba histolytica in Microcultures from Inocula of Single Trophozoites in Cell-Free Medium Prepared from Embryos of the ChickJournal of Parasitology, 1957
- ELECTROPHORETIC AND ULTRACENTRIFUGAL ANALYSES OF PROTEIN EXTRACTED FROM WHOLE MAMMALIAN MUSCLESJournal of Biological Chemistry, 1949