MONOCLONAL-ANTIBODY LOCALIZATION OF A-ISOANTIGEN AND B-ISOANTIGEN IN NORMAL AND MALIGNANT FIXED HUMAN-TISSUES

  • 1 January 1984
    • journal article
    • research article
    • Vol. 117  (3) , 451-461
Abstract
The expression of human blood group A and B isoantigens in normal and malignant tissues from stomach, colon and pancreas was analyzed in an immunoperoxidase assay using monoclonal antibodies specific for these isoantigens. Appropriate isoantigen expression was demonstrated in the normal epithelium from the stomach, pancreas and proximal but not distal colon of blood group A, AB or B patients. Half of all gastric carcinomas and of proximal colon carcinomas showed complete loss of isoantigen, whereas the adjacent mucosa in these cases continued to express appropriate isoantigen. Isoantigen expression was completely lost in only 13% of pancreatic carcinomas tested. Neither A nor B isoantigen was detected in normal epithelium from the distal colon. By contrast, 85% of carcinomas derived from this site showed reexpression of isoantigen. Inappropriate expression of A isoantigen was detected in pancreatic carcinomas (2/5) but not in gastric or colon carcinomas (0/21). Inappropriate expression of B substance was not detected in any tissue (0/38). Interestingly, differential binding of antibodies to Type 1 vs. Type 2 and/or difucosyl vs. monofucosyl blood group B substances was manifested by differences in intensity of staining for endothelium and red blood cells.

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