CULTURAL AND BIOCHEMICAL CRITERIA FOR IDENTIFICATION OF HEMOPHILUS-SPP FROM SWINE

  • 1 January 1977
    • journal article
    • research article
    • Vol. 38  (1) , 7-11
Abstract
Haemophilus cultures (22) of types prevalent in swine and of different geographic origins were subjected to biochemical and cultural examinations. Three subgroups were identified: 1 was urease-positive, produced porphyrin from .delta.-aminolevulinic acid, and grew on infusion mediums supplemented only with V factor; the 2nd was urease-negative, porphyrin-positive, and grew only on serum-enriched media with added V factor; and the 3rd was urease-negative, porphyrin-negative, and grew only on serum-enriched media with added V and X factors. The groups generally corresponded to H. parahaemolyticus, H. parasuis and H. suis, respectively. By urease and porphyrin tests it was possible to assign, presumptively, porcine Haemophilus cultures to 1 of the 3 spp. Other tests, such as .beta.-galactosidase, hemolysis and fermentation of carbohydrates were of secondary value in differentiating between these species.

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