The effects of surface chemistry and adsorbed proteins on monocyte/macrophage adhesion to chemically modified polystyrene surfaces
- 26 July 2001
- journal article
- research article
- Published by Wiley in Journal of Biomedical Materials Research
- Vol. 57 (3) , 336-345
- https://doi.org/10.1002/1097-4636(20011205)57:3<336::aid-jbm1176>3.0.co;2-e
Abstract
Monocytes and macrophages play critical roles in inflammatory responses to implanted biomaterials. Monocyte adhesion may lead to macrophage activation and the foreign body response. We report that surface chemistry, preadsorbed proteins, and adhesion time all play important roles during monocyte adhesion in vitro. The surface chemistry of tissue culture polystyrene (TCPS), polystyrene, Primaria®, and ultra low attachment (ULA) used for adhesion studies was characterized by electron spectroscopy for chemical analysis. Fibrinogen adsorption measured by 125I-labeled fibrinogen was the lowest on ULA, higher on TCPS, and the highest on polystyrene or Primaria. Monocyte adhesion on protein preadsorbed surfaces for 2 h or 1 day was measured with a lactate-dehydrogenase method. Monocyte adhesion decreased over time. The ability of preadsorbed proteins to modulate monocyte adhesion was surface dependent. Adhesion was the lowest on ULA, higher and similar on TCPS or polystyrene, and the highest on Primaria. Monocyte adhesion on plasma or fibrinogen adsorbed surfaces correlated positively and linearly to the amount of adsorbed fibrinogen. Preadsorbed fibronectin, immunoglobulin G, plasma, or serum also promoted adhesion compared with albumin preadsorbed or uncoated surfaces. Overall, biomaterial surface chemistry, the type and amount of adsorbed proteins, and adhesion time all affected monocyte adhesion in vitro. © 2001 John Wiley & Sons, Inc. J Biomed Mater Res 57: 336–345, 2001Keywords
This publication has 45 references indexed in Scilit:
- Human monocyte/macrophage adhesion, macrophage motility, and IL-4-induced foreign body giant cell formation on silane-modified surfacesin vitroJournal of Biomedical Materials Research, 1998
- Leukocyte-polytetrafluoroethylene interaction enhances proliferation of vascular smooth muscle cells via tumor necrosis factor-α secretionKidney International, 1997
- Human monocyte/macrophage adhesion and cytokine production on surface‐modified poly(tetrafluoroethylene/hexafluoropropylene) polymers with and without protein preadsorptionJournal of Biomedical Materials Research, 1995
- Comparative Cytokine Release from Human Monocytes, Monocyte-Derived Immature Mast Cells, and a Human Mast Cell Line (HMC-1)Journal of Investigative Dermatology, 1994
- LFA-3, CD44, and CD45: Physiologic Triggers of Human Monocyte TNF and IL-1 ReleaseScience, 1990
- The Effects of Surface Chemistry and Coagulation Factors on Fibrinogen Adsorption from PlasmaaAnnals of the New York Academy of Sciences, 1987
- Phagocytosis and inflammatory stimuli induce GM-CSF mRNA in macrophages through posttranscriptional regulationCell, 1987
- Secretory products of macrophages.Journal of Clinical Investigation, 1987
- Quantitative characterization of cells at the interface of long‐term implants of selected polymersJournal of Biomedical Materials Research, 1986
- Macrophage spreading in vitroExperimental Cell Research, 1973