An improved technique for the study of immunofluorescence using non-decalcified frozen guinea pig cochlea

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Abstract
Summary A technique for producing frozen sections from the non-decalcified guinea pig cochlea is described. The sections can be utilised or various immunofluorescent studies, including the detection of humoral anti-bodies against different cellular elements in patients with cochleo-vestibular disorders. The technique assures the preservation of the cochlear architecture and the tissue antigenicity, and it avoids damage to the fine cochlear structural details.

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