Isolation and Preliminary Characterization of Temperature-sensitive Mutants of the Murine Sarcoma Leukaemia Virus Complex

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Abstract
Five temperature-sensitive mutants (ts 1-5) were isolated from a stock of the Moloney strain of murine sarcoma leukemia virus complex which had been mutagenized by UV irradiation or N-methyl-N''-nitro-N-nitrosoguanidine. In mouse cells at the non-permissive temperature the mutants formed fewer foci of transformed cells than at the permissive temperature. The ts mutants were characterized by testing: murine leukemia virus (MuLV) clones from the ts complex, the effect of additional wild type MuLV on focus formation, focus formation in rat cells and focus formation with pseudotypes rescued from non-producer cells. Two mutants (ts 1 and ts 3) were ts MuLV which did not possess heat labile virion proteins and were not ts in post-penetration helper functions necessary for the fixation of sarcoma virus transformation. The remaining 3 mutants (ts 2, ts 4 and ts 5) were ts murine sarcoma viruses which showed no temperature-sensitive effect on the maintenance of transformed cell morphology or on colony forming efficiency in soft agar.

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