The in vitro Modification of Phosphorylated Pyruvate Kinase by a Ca(2+)-activated Protease from Rat Liver.
- 1 January 1980
- journal article
- research article
- Published by Danish Chemical Society in Acta Chemica Scandinavica
- Vol. 34b (6) , 419-422
- https://doi.org/10.3891/acta.chem.scand.34b-0419
Abstract
A Ca2+-activated protease from rat liver cell sap was prepared. It acted on rat liver pyruvate kinase that had been phoshorylated by the catalytic subunit of cAMP-dependent protein kinase, the activity being optimum at neutral pH. The modified pyruvate kinase had the same Vmax as the phosphoenzyme, but showed a lower affinity for the substrate phosphoenolpyruvate. The possibility that this proteolytic attack is the step that initiates further degradation in the cell is discussed.This publication has 13 references indexed in Scilit:
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