EFFECTS OF ACTINOMYCIN-D AND PUROMYCIN ON INDUCTION OF CYTOSOL ENZYMES INVOLVED IN ANDROGEN METABOLISM BY TESTOSTERONE IN FEMALE RAT-LIVER

  • 1 January 1977
    • journal article
    • research article
    • Vol. 69  (1) , 1-10
Abstract
Induction of the cytosol enzymes involved in the androgen metabolism in the female rat liver by testosterone was studied. The treatment with testosterone propionate [TP] increased the production of 5.beta.-androstane-3.alpha.,17.beta.-diol in the 105,000 .times. g supernatant when 4-14C-testosterone was used as a substrate. The conversion of 4-14C-androstenedione to labeled testosterone was increased by the injection of TP into female rats. Actinomycin D or puromycin administered for 4 days with daily s.c. injections of TP prevented the increase of these enzymes induced by TP. Actinomycin D given once with the final injection of TP blocked the induction of cytosol 17.beta.-hydroxysteroid dehydrogenase by the treatment of TP, whereas puromycin given in the same manner as actinomycin D had no such blocking effect on the enzyme. Both antibiotics given once with the final injection of TP showed no preventive effects on the induction of cytosol .DELTA.4-5.beta.-reductase by TP.