Purification and Characterisation of a Small DNA‐Binding Protein, HB, from Bacillus globigii
- 3 March 1982
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 122 (3) , 627-632
- https://doi.org/10.1111/j.1432-1033.1982.tb06485.x
Abstract
A small, heat-stable DNA-binding protein was purified from B. globigii: HB protein. The protein binds cooperatively to double-stranded DNA and the DNA-protein complexes are destabilized by the presence of moderate (0.1 M) levels of monovalent cations or by low levels (10 mM) of Mg2+. Two small DNA-binding proteins from Escherichia coli, NS1 and NS2, also known as HU protein, also were purified. Antibodies against all 3 proteins were prepared. All 3 are, by immunological criteria, related.This publication has 20 references indexed in Scilit:
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