Modification of Infectious Bovine Rhinotracheitis (IBR) Virus in Tissue Culture and Development of a Vaccine

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Abstract
Virulent IBR virus was modified by rapid passages and terminal dilutions in bovine embryo kidney tissue culture to the extent that it could be injected into cattle without production of disease, but with formation of specific antibodies. The avirulence of this modified virus was demonstrated by intramuscular and intranasal inoculation of susceptible cattle. In addition, it was shown that the virus did not spread from intramuscularly-inoculated cattle to susceptible contacts, and could not be reisolated from blood or nasal washings of vaccinated cattle, making the possibility of reversion to virulence by natural back-passages very unlikely. This modified virus prepared in tissue culture can be lyophilized, thus facilitating its use as a vaccine for prevention of IBR disease.
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