Electron microscopic morphometric analysis of small cortical and medullary thymocytes from the rat

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Abstract
Summary Electron microscopic morphometric analysis of rat small thymocytes reveals quantitative differences between small cortical and medullary thymocytes. The unit gravity velocity sedimentation technique was used to obtain a cellular pool composed mainly of small sized thymocytes. Stimulation “in vitro” with phytohemagglutinin followed by cell size separation was employed to separate cortical small thymocytes. Furthermore, isolation of medullary small thymocytes was carried out by treatment “in vivo” with hydrocortisone. Our results show that the majority of the quantitative changes correspond to differences in the distribution of chromatin and the density of perichromatin granules. They demonstrate the importance of chromatin pattern analysis for the identification of small cortical and medullary thymocytes.