Assignment of a porcine male-specific DNA repeat to Y-chromosomal heterochromatin
- 1 January 1992
- journal article
- Published by S. Karger AG in Cytogenetic and Genome Research
- Vol. 61 (2) , 152-154
- https://doi.org/10.1159/000133395
Abstract
Primers were designed to amplify by PCR a 509-bp genomic fragment from male pig DNA, using the porcine male-specific repeat sequence described by McGraw et al. (1988). This PCR product showed male-specific hybridization in Southern blots. Nonradioactive in situ hybridization localized it to the entire length of the heterochromatic portion of Yq. The assignment was confirmed using the PCR primer pDYZ1-S for primed in situ labeling.Keywords
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