A 25 kDa α2‐microglobulin‐related protein is a component of the 125 kDa form of human gelatinase
- 17 December 1992
- journal article
- Published by Wiley in FEBS Letters
- Vol. 314 (3) , 386-388
- https://doi.org/10.1016/0014-5793(92)81511-j
Abstract
Besides the monomeric mammalian 95 kDa progelatinase, two additional forms, a disulfide-bridged 220 kDa dimer and a 125 kDa form were isolated from human PMN leukocytes. The 125 kDa progelatinase was identified as a covalently linked, disulfide-bridged heterodimer formed of the monomer with a 23 kDa protein. This 25 kDa protein was isolated from gelatinase bound to the affinity support of gelatin-Sepharose and eluted by DTE-containing buffer. The amino acid sequence of tryptic peptides of this protein revealed homology with an α2-microglobulin-related protein from rats, a protein so far unknown in humans.Keywords
This publication has 21 references indexed in Scilit:
- Characterization of Neutral Proteinases from Alzheimer‐Affected and Control Brain Specimens: Identification of Calcium‐Dependent Metalloproteinases from the HippocampusJournal of Neurochemistry, 1992
- Mercurial activation of human PMN leucocyte type IV procollagenase (gelatinase)FEBS Letters, 1992
- The secretion of the tissue inhibitor of metalloproteinases (TIMP) by human synovial fibroblasts is modulated by all-trans-retinoic acidBiochimica et Biophysica Acta (BBA) - Molecular Cell Research, 1991
- The complex between a tissue inhibitor of metalloproteinases (TIMP‐2) and 72‐kDa progelatinase is a metalloproteinase inhibitorEuropean Journal of Biochemistry, 1991
- Evidence for the involvement of type II domains in collagen binding by 72 kDa type IV procollagenaseFEBS Letters, 1991
- Structural and functional characterization of complement c8γ, a member of the lipocalin protein familyMolecular Immunology, 1991
- A comparison of male rat and human urinary proteins: Implications for human resistance to hyaline droplet nephropathyToxicology and Applied Pharmacology, 1990
- Molecular Cloning of Odorant-Binding Protein: Member of a Ligand Carrier FamilyScience, 1988
- Purification of a Type V Collagen Degrading Metalloproteinase from Rabbit Alveolar MacrophagesCollagen and Related Research, 1984
- STRUCTURAL AND FUNCTIONAL STUDIES OF VITAMIN A‐BINDING PROTEINS*Annals of the New York Academy of Sciences, 1981