Leukocyte Pyruvate Kinase Expression is Reduced in Normal Human Pregnancy but not in Pre‐eclampsia
- 6 July 2010
- journal article
- research article
- Published by Wiley in American Journal of Reproductive Immunology
- Vol. 64 (2) , 137-151
- https://doi.org/10.1111/j.1600-0897.2010.00881.x
Abstract
Citation Xu Y, Madsen‐Bouterse SA, Romero R, Hassan S, Mittal P, Elfline M, Zhu A, Petty HR. Leukocyte pyruvate kinase expression is reduced in normal human pregnancy but not in pre‐eclampsia. Am J Reprod Immunol 2010; 64: 137–151 Problem Emerging evidence suggests that metabolism influences immune cell signaling and immunoregulation. To examine the immunoregulatory role of glycolysis in pregnancy, we evaluated the properties of pyruvate kinase in leukocytes from non‐pregnant women and those with normal pregnancy and pre‐eclampsia. Method of study We evaluated pyruvate kinase expression in lymphocytes and neutrophils from non‐pregnant, pregnant, and pre‐eclampsia patients using fluorescence microscopy and flow cytometry. Leukocyte pyruvate kinase activity and pyruvate concentrations were also evaluated. To study pyruvate’s effect on signaling, we labeled Jurkat T cells with Ca2+ dyes and measured cell responses in the presence of agents influencing intracellular pyruvate. Results The expression of pyruvate kinase is reduced in lymphocytes and neutrophils from normal pregnant women in comparison with those of non‐pregnant women and pre‐eclampsia patients. Similarly, the activity of pyruvate kinase and the intracellular pyruvate concentration are reduced in leukocytes of normal pregnant women in comparison with non‐pregnant women and women with pre‐eclampsia. Using Jurkat cells as a model of leukocyte signaling, we have shown that perturbations of intracellular pyruvate influence Ca2+ signals. Conclusion Normal pregnancy is characterized by reduced pyruvate kinase expression within lymphocytes and neutrophils. We speculate that reduced pyruvate kinase expression modifies immune cell responses due to reduced pyruvate concentrations.Keywords
This publication has 87 references indexed in Scilit:
- Leukocytes of pregnant women with small-for-gestational age neonates have a different phenotypic and metabolic activity from those of women with preeclampsiaThe Journal of Maternal-Fetal & Neonatal Medicine, 2010
- A sensitive fluorimetric assay for pyruvateAnalytical Biochemistry, 2009
- mTOR regulates memory CD8 T-cell differentiationNature, 2009
- Evidence of changes in the immunophenotype and metabolic characteristics (intracellular reactive oxygen radicals) of fetal, but not maternal, monocytes and granulocytes in the fetal inflammatory response syndromejpme, 2009
- Enhancing CD8 T-cell memory by modulating fatty acid metabolismNature, 2009
- An enzymatic fluorimetric assay for glucose-6-phosphate: Application in an in vitro Warburg-like effectAnalytical Biochemistry, 2009
- Glucose metabolism in lymphocytes is a regulated process with significant effects on immune cell function and survivalJournal of Leukocyte Biology, 2008
- Lactate and malignant tumors: A therapeutic target at the end stage of glycolysisJournal of Bioenergetics and Biomembranes, 2007
- Effects of overexpression of the liver subunit of 6-phosphofructo-1-kinase on the metabolism of a cultured mammalian cell lineBiochemical Journal, 2000
- Bidirectional cytokine interactions in the maternal-fetal relationship: is successful pregnancy a TH2 phenomenon?Immunology Today, 1993