Improved Fluorescence Assay of Liposome Lysis

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Abstract
A simple fluorescence assay for liposome stability is described. A fluorogenic enzyme substrate, 4-methylumbelliferyl-.beta.-D-glucuronide, is encapsulated in liposomes. In the presence of Escherichia coli .beta.-glucuronidase, any of the substrate that has been released into the medium is converted to 4-methylumbelliferone, which is monitored fluorimetrically. The method offers several advantages over current methods, including high gain, extremely low leakage, and facile quantitation. The use of the assay as part of a prototype immunoassay is described.