Analyse des corps proteiques isolés de Lablab purpureus (L.) Sweet: Localisation intracellulaire des globulines, protéases et inhibiteurs de la trypsine

Publisher Website
Google Scholar
Abstract
Cotyledonary cells are submitted to fractionation by isopycnic centrifugation. Small intact protein bodies are collected in the densest zones (d=1.205–1.237 g/cm3). Fragments of larger bodies are gathered in zones of lower density (d=1.205 g/cm3). Small dense bodies are largely sedimentable after dilution, whereas fragments of the large bodies dissociate into a dense sedimentable clot and into floating elements which contain most of the globulins and all of the albumins. Among the dissociated floating components are the BAPA-active endopeptidases and the trypsin inhibitors (BAPA=α-N-benzoyl-DL-arginine-p-nitroanilide). A caseolytic activity remains with the dense mass. The localisation of the albumins, globulins, proteases and trypsin inhibitors is discussed. Relations between solubility, structure and function are considered.