Enzyme‐linked immunosorbent (ELISA) determination of aflatoxin B1in peanut butter: Collaborative trial
- 1 October 1988
- journal article
- research article
- Published by Taylor & Francis in Food Additives & Contaminants: Part A
- Vol. 5 (4) , 601-608
- https://doi.org/10.1080/02652038809373724
Abstract
Fourteen laboratories in the United Kingdom participated in a collaborative trial of a commercially available ELISA test kit for the determination of aflatoxin B1 in peanut butter. Each laboratory carried out six replicate analyses of each of six individual samples. Collaborators received a control, uncontaminated sample, together with samples prepared by blending naturally‐contaminated and control material to give target levels of 8 μg/kg, 25 μg/kg and 75 μg/kg. Two of these samples (8 μg/kg and 25 μg/kg) were supplied as undisclosed duplicates. The repeatabilities of the assay ranged from 6.2 to 16.7 μg/kg. The reproducibilities for aflatoxin B1 concentrations in naturally‐contaminated samples ranged from 3.6 to 18.7 μg/kg using uncontaminated peanut butter as a reference blank. Modifications to the format of the commercial kit were recommended as a result of the collaborative trial.Keywords
This publication has 3 references indexed in Scilit:
- A survey of the occurrence of aflatoxin B1in peanut butters by enzyme‐linked immunosorbent assayFood Additives & Contaminants: Part A, 1988
- A survey of aflatoxins in peanut butters, nuts and nut confectionery products by HPLC with fluorescence detectionFood Additives & Contaminants: Part A, 1985
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