Solid Phase Extraction/High Performance Liquidchromatography Method for the Determination of Methyl Anthranilate Residues in Blueberries

Abstract
Methyl anthranilate fortified blueberries were extracted with methanol, cleaned up by solid phase extraction and analyzed by reverse phase high performance liquid chromatography. Methyl anthranilate was quantified by UV absorbance and fluorescence detection. Recoveries of 70 ± 13% and 14% were obtained for blueberry samples fortified with methyl anthranilate at 5 and 0.15 ppm respectively. The method limits of detection for ultraviolet and fluorescence detection were 0.04 and 0.009 μg methyl anthranilate/g, respectively.

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