Plantlets from somatic callus tissue of the East Indian Rosewood (Dalbergia latifolia Roxb.)
- 1 June 1986
- journal article
- research article
- Published by Springer Nature in Plant Cell Reports
- Vol. 5 (3) , 199-201
- https://doi.org/10.1007/bf00269118
Abstract
Callus-mediated shoot bud formation was demonstrated in Dalbergia latifolia Roxb. (East Indian Rosewood). Cultures were raised from shoot explants of six year-old plants on Murashige and Skoog (MS) medium supplemented with naphthaleneacetic acid (NAA) and benzyladenine (BA). A sequential treatment of callus with increasing BA levels and decreasing NAA ensured shoot bud induction. Rooting of shoots was achieved by a three-step culture procedure involving 1) White's(W) liquid medium containing indoleacetic acid (IAA), naphthaleneacetic acid and indolebutyric acid (IBA), 2) half-strength MS agar-solidified medium with charcoal (0.25%) and 3) half-strength MS liquid medium.Keywords
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