COAGULATION STUDIES OF PLASMAS FROM HEALTHY DOMESTICATED ANIMALS AND PERSONS

  • 1 January 1981
    • journal article
    • research article
    • Vol. 42  (12) , 2170-2177
Abstract
Optimal reaction conditions for the activated partial thromboplastin time (APTT), 1-stage prothrombin time (PT) and Russell''s viper venom time were studied for pooled plasmas of horses, cattle, sheep, goats, swine, dogs, cats and persons. Changes in CaCl2 and NaCl concentrations had significant effects on the APTT and PT reactions. The APTT was more sensitive than the PT to changes in CaCl2 concentration. The CaCl2 concentration recommended by the manufacturer for the APTT was suboptimal for some plasmas of domesticated animals. Infusorial earth (Celite, activator) concentration provided by the manufacturer was optimal for plasmas of all species, with exception of horses and swine. Optimal incubation times for the APTT for the equine, bovine, ovine and caprine plasmas were shorter than the 5 min recommended for human plasma. Optimal incubations for porcine and canine plasmas were longer than 5 min. The APTT and PT of all plasmas were stable for up to 3 h storage at 4.degree. C in all species'' plasmas tested with the exception of the porcine plasma.