The Orientation of Certain Root-nodule Bacteria at Interfaces, Including Legume Root-hair Surfaces

Abstract
Adhesion of rhizobia was examined, using sterile subterranean clover (Trifolium subterraneum), pea (Pisum sativum) and soybean (Glycine max). A perpendicular orientation was shown for Rhizobium trifolii on subterranean clover. The pole of each bacterium was attached to the root hair surface by some form of bridging polymer. An identical mode of attachment was observed on the surface of Spurr''s medium. The mode of attachment may provide a means for the localization of bacterial products concerned with some phase of the infection process.

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