On the Relation between Nitrogen Fixation and Leghaemoglobin Content of Leguminous Root Nodules.

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Abstract
Certain strains of legume bacteria were found to form nodules on the roots of the host plant, but the nodules did not fix N2. Comparison between the nodules formed by these strains and those of effective strains showed that a red pigment, leghaemoglobin, was always present in the effective nodules. Leghaemoglobin has been purified to a degree that its iron content (0.29%) was the same as that of the haemoglobin of blood. A parallelism between the leghaemoglobin content of pea nodules and their N-fixing ability seems to exist. N-fixation has not been accomplished with the free-living legume bacteria in nutrient solutions to which had been added either raw extract of nodules containing leghaemoglobin, legmethaemoglobin or purified prepns. of these chromo-proteins. A chronological description of the process of fixation is given.

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