Fertility Test of Frozen Boar Semen

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Abstract
In experiment 1, the semen was frozen in tris-fructose-EDTA or B[Beltsville]F3 diluents at 0.25 .times. 109/ml sperm concentration and extended after thawing with either seminal plasma (SP) or the freezing medium (FM) containing no cryoprotective agent. In the 2nd experiment, the semen was glycerolated by 2 methods, frozen at 1.0 .times. 109/ml sperm concentration, and extended with FM before insemination. Fertility, after double insemination within 1 estrus with semen frozen in tris-fructose-EDTA or BF3 diluents, varied depending on the medium used for extension of thawed semen. The farrowing rates for semen frozen in the former diluent with FM and SP post-thawing media were 4/8 and 1/8, respectively, and for semen frozen in BF3 diluent with FM and SP post-thawing extenders, 1/8 and 5/8, respectively. The mean farrowing for the 32 animals inseminated was 34.4%. Pregnancies for semen frozen in tris-fructose-EDTA and glycerolated at 30 or 5.degree. C were 5/12 and 4/12, respectively, and for single and double inseminations, 6/12 and 3/12, respectively. Of 24 animals inseminated 37.5% farrowed.

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